High sensitivity and low detection limit (0.5 mg/dL)
- A rapid (15 minutes) and robust enzyme-based assay which does not require expensive instrumentation
- High reproducibility
Creatinine Assay Kit for Measuring Creatinine Levels in the Serum
The MaxDiscovery™ Creatinine Assay Kit is a plate-based colorimetric assay for the determination of creatinine in serum samples.
The MaxDiscovery Creatinine Assay Kit is a simple, direct and automation-compatible method for measuring creatinine levels in the serum. This kit uses a direct colorimetric reaction scheme. At high pH, creatinine specifically reacts with picrate reagent to form a red-colored complex. The absorbance measured at 510 nm, is proportional to the concentration of creatinine in the sample. This creatinine assay kit also comes with a control solution containing a creatinine standard (20 mg/dL) which can be used to calibrate the assay and generate a standard curve.
Creatinine is a waste product formed throughout the body and removed from circulation through the kidneys. Creatinine levels in the blood are related to excretion rate by the kidneys. Creatinine levels in blood increase when the kidneys are diseased or damaged. For this reason elevated serum creatinine levels are a highly specific marker for kidney disease.
Publications Citing the Use of the MaxDiscovery Creatinine Assay Kit
Patlolla, A. K., Randolph, J., Kumari, S. A. and Tchounwou, P. B. (2016) Toxicity Evaluation of Graphene Oxidein Kidneys of Sprague-Dawley Rats. Int. J. Environ. Res. Public Health. 13:4. 380. doi: 10.3390/ijerph13040380.
Zhu, D., Lam, D. H., Purwanti, Y. I., Goh, S. L., Wu, C., Zeng, J., Fan, W. and Wang, S. (2013) Systemic delivery of fusogenic membrane glycoprotein-expressing neural stem cells to selectively kill tumor cells. Mol. Ther. 21:8, 1621-30.
The MaxDiscovery Creatinine Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The shelf life is 6 months after receipt when the kit is properly stored.