Increased sensitivity with low detection limit (20 U/L)
- Robust kinetic assay
- Does not require expensive instrumentation
- Highly reproducible results
- Requires only 5 µL of serum
- Measure absorbance in UV spectrum (340 nm)
The MaxDiscovery™ Lactate Dehydrogenase (LDH) Enzymatic Assay Kit is a colorimetric plate-based assay to directly determine the amount of Lactate Dehydrogenase enzyme in samples. This kit enables biomedical researchers to determine LDH levels in serum from rodents, such as mice and rats, and other vertebrates including fish. The assay utilizes a simple, proven colorimetric (UV) enzymatic assay to specifically detect LDH. It provides accurate results even in complex liquid mixtures.
Lactate Dehydrogenase (LDH) is a ubiquitously-expressed intracellular enzyme which catalyzes the reversible oxidation of lactate to pyruvate. LDH is one of the most clinically important protein markers in serum because its level changes in response to a number of health-related states. For example, elevated LDH serum levels are often caused by heart, liver and kidney disease as well as in numerous types of cancer. Also, the presence of elevated levels of the enzyme in serum after administration of drugs and experimental therapeutic agents is associated with organ toxicity. Therefore, monitoring serum levels of LDH enzyme has become a routine and fundamental means to monitor organ toxicity.
Selected References Which Cite the Use of the MaxDiscovery LDH Enzymatic Assay Kit:
Kang, S. J., (2016) Chemically induced hepatotoxicity in human stem cell-induced hepatocytes compared with primary hepatocytes and HepG2. Cell Biology and Toxicology. 1-15. doi: 10.1007/s10565-016-9342-0.
Roubille, F., et al. (2011) Delayed Postconditioning in the Mouse Heart In Vivo. Circulation, 124: 1330-1336.
MaxDiscovery Lactate Dehydrogenase Enzymatic Assay Kit Specs
The MaxDiscovery Lactate Dehydrogenase (LDH) Enzymatic Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for serially-diluted standards). Upon receipt of the kit, store the standards and buffer at -20°C and the remainder of the kit at 4°C. Once the LDH Reagent Mix is reconstituted the shelf life of the kit is 3 months when properly stored.