- High sensitivity and low detection limit (1 mg/dL)
- Rapid (5 minute) and robust colorimetric assay
- Does not require expensive instrumentation.
- High reproducibility
Rapid Assay for the Determination of Bilirubin in Serum
The MaxDiscovery™ Total Bilirubin Assay Kit is a plate-based colorimetric method for the determination of bilirubin in serum samples. This kit uses a simple and direct spectrophotometric assay to detect bilirubin directly, enabling researchers to detect bilirubin levels in animal serum. The MaxDiscovery™ Total Bilirubin Assay Kit uses an aqueous diazotized sulfanilic acid reagent to modify bilirubin in the sample to an azobilirubin form which absorbs light at 550 nm. The absorption measured at 550 nm, is proportional to the concentration of total bilirubin in the sample. The kit contains sufficient materials to test 42 serum samples in duplicate. The kit also contains a control solution containing a standard (equivalent to 20 mg/dL bilirubin) which can be used to calibrate the assay and verify kit performance.
Bilirubin is a metabolite (breakdown product) of hemoglobin present in the bloodstream and liver of all mammals. In the liver, bilirubin is converted into a water soluble form (known as “direct bilirubin”). The determination of serum bilirubin is an important marker for the diagnosis of several diseases; elevated levels of bilirubin are strongly associated with hemolysis, blockage of the biliary tract, and liver disease.
Selected Publications that Cite Use of the MaxDiscovery™ Total Bilirubin Assay Kit:
Adejo, G. O., Atawodi, S. E., Ameh, D. A. and Ibrahim, S. (2014) Anti-Peroxidative, Protective and Ameliorative Properties of Methanol Extract of All Parts of Morinda Lucida Benth in CCl4-Induced Liver Injury. Nat Prod Chem Res S1:003. doi: 10.4172/2329-6836.S1-003.
Omeh, N. Y., Ejiofor, E. U., Echeme, M. A., Nwoko, C. O. (2015) Evaluation of the Serum Liver Enzymes Markers, Lipid Profile and Kidney Function Parameters in Typhoid Patients. International Journal of TROPICAL DISEASE & Health. 8(2): 79-89.
Pratap, A., et al. (2011) Cyclopamine Attenuates Acute Warm Ischemia Reperfusion Injury in Cholestatic Rat Liver: Hope for Marginal Livers. Mol. Pharmaceutics. 8 (3), pp 958–968. doi: 10.1021/mp200115v.
Saidu, O. Y. et al. (2014) Liver Status and Oxidative Stress Activity of Wistar Albino Rat Fed Parkia Biglobosa Seeds. Sch. J. App. Med. Sci. 2(4B):1290-1294.
MaxDiscovery Total Bilirubin Assay Kit Specs
The MaxDiscovery Total Bilirubin Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). Upon receipt, store kit at 4 °C. The shelf life is 6 months when the kit is properly stored.