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For the determination of uric acid in serum
The MaxDiscovery™ Uric Acid Assay Kit is a simple, direct and automation-compatible method for measuring uric acid levels in serum samples from any mammal. This kit uses a coupled reaction scheme: Uric acid is first transformed by uricase enzyme to form allatoin, carbon dioxide and hydrogen peroxide. In the second step, the hydrogen peroxide reacts with a chromogenic dye using peroxidase to form a visibly-colored (red) dye product. The amount of dye product, measured by the sample absorbance at 520 nm, is proportional to the concentration of uric acid in the sample. The kit includes a control solution containing a uric acid standard (20 mg/dL), which is used to calibrate the assay.
Uric acid is an important end-product metabolite of purine metabolism. Uric acid produced from purine degradation is excreted through the kidneys. The determination of serum uric acid is an important marker for a number of disease states. Elevated uric acid levels are strongly associated with gout, renal failure, and other diseases such as myeloproliferative disorders.