High sensitivity and low detection limit (<10 U/L)
- A rapid (10 minute), robust enzyme-based assay which does not require expensive
- High reproducibility
- Only requires 10 μL of serum
The MaxDiscovery™ gamma-Glutamyl Transferase (GGT) Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the gamma-glutamyl transferase enzyme in 10 µL of serum from rodents or other mammals. gamma-glutamyl transferase (GGT) is a metabolic enzyme expressed primarily in the liver, kidneys and other organs. Organ damage, especially damage to the liver, causes the release of this enzyme into the blood. Elevation of GGT levels is often an indication of liver damage and has been associated with liver injury as well as pancreatic and myocardial disorders. GGT is also a very useful tool for preclinical investigation of experimental drug formulations and GGT levels are commonly used to monitor and attenuate the toxic effects of experimental drug formulations in rodents. The kit uses a spectrophotometric, kinetic assay to detect changes in gamma-glutamyl transferase levels directly from serum samples.
The MaxDiscovery g-Glutamyl Transferase (GGT) Enzymatic Assay Kit uses an enzymatic reaction to measure enzyme levels in serum. The assay measures the cleavage of a specific GGT substrate (g-glutamyl-p-ntiroanilide) by the enzyme. The production of the p-nitroaniline(pNA) product, measured at 405 nm, is proportional to the level of GGT enzyme in the sample.
The absorbance of each sample well at 405 nm is measured using a plate reader. The concentration of GGT in each sample is then directly determined from the change in absorbance at 405 nm within 10 minutes. Dilutions of the pNA Control, included in the kit, can be used to construct a standard curve to calibrate the assay and confirm assay linearity.
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Bility MT, et al. (2014) Hepatitis B Virus Infection and Immunopathogenesis in a Humanized Mouse Model: Induction of Human-Specific Liver Fibrosis and M2-Like Macrophages. PLoS Pathog 10(3): e1004032. doi:10.1371/journal.ppat.1004032.
Sampaziotis, F. et al. (2015) Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nature Biotechnology. doi:10.1038/nbt.3275.
The MaxDiscovery gamma-Glutamyl Transferase (GGT) Enzymatic Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. Store the kit at 4°C. The shelf life of the kit is 12 months when properly stored. Once the GGT Reagent Mix is reconstituted the shelf life of the kit is 6 months when properly stored.