- High sensitivity and large assay range (1 - 8 mmol/L)
- A rapid (15 minute) and robust enzyme-based assay which does not require expensive instrumentation
- High reproducibility
Highly Sensitive Fructosamine Assay For the Determination of Fructosamine in Serum and Tissue
The MaxDiscovery™ Fructosamine Enzymatic Assay Kit is a plate-based colorimetric assay for the determination of Fructosamine in serum samples. The kit uses a spectrophotometric assay to detect Fructosamine directly from serum samples, enabling researchers to detect Fructosamine levels in animal serum and other tissue matrices.
The MaxDiscovery Fructosamine Enzymatic Assay Kit is a simple, direct and automation- compatible method for measuring fructosamine levels in serum. This kit is based on the ability of ketoamines to directly reduce nitroblue tetrazolium (NBT) to colored formazan dye under alkaline conditions. The absorbance measured at 550 nm, is proportional to the concentration of fructosamine in the sample. The kit also comes with a control solution containing a fructosamine standard which can be used to calibrate the assay.
Fructosamines are stable carbohydrate-protein complexes that form in the blood by glycation reactions between sugars and primary amines. In diabetes, blood sugars are measured by either monitoring current blood glucose level or by glycated hemoglobin (HbA1c). Similar to the HbA1c test, fructosamine testing calculates the fraction of total serum proteins that have undergone glycation and typically reflects albumin glycation. Since the half-life of albumin is approximately 20 days, the fructosamine level reflects 1-2 week changes in blood glucose. Therefore, fructosamine testing provides earlier and more sensitive detection for diabetes than many other carbohydrate tests.
MaxDiscovery Fructosamine Assay Kit Specs
The MaxDiscovery Fructosamine Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). Store the kit at 4 °C. The shelf life is 6 months after receipt when the kit is properly stored. After opening, the Fructosamine Standard is stable for 30 days stored at 4°C. Reconstituted Fructosamine Reagent is stable for 30 days if stored refrigerated at 4°C.