AuroFlow™ PR1ME™ BT Combo MRL Assay

Catalog# Product Name Quantity US List Price
1134-01 AuroFlow™ PR1ME BT Combo MRL Assay 50 strips Inquire
1134-02 AuroFlow™ PR1ME™ BT Combo MRL Assay 100 strips Inquire

These kits are available for purchase by email, fax or phone only. Please email your order to, fax it to 1-512-707-8122 or call 512-707-8993.

  • Rapid strip test method – 7 minutes
  • Detects 17 beta-lactams (including select metabolites) below EU MRL (maximum residue limit)
  • Detects 3 tetracyclines under EU regulatory limits
  • Easy visual test interpretation


Rapid Strip Test for Beta-lactam and Tetracycline Antibotic Residues in Milk

The AuroFlow™ PR1ME BT Combo MRL Assay is a qualitative and rapid lateral flow assay designed for the detection of a broad range of antibiotics in raw, commingled cow's milk. These rapid tests detect 17 beta-lactams plus metabolites and 3 tetracyclines below EU MRL requirements in a one-step process. The fast, simplified protocol can be seen in the next tab. Results of the test can be interpreted visually. If any test line on the strip is less intense than the control line, the sample is positive for that antibiotic.

Beta-lactam antibiotics are a class of broad-spectrum antibiotics, consisting of all antibiotic agents that contain a beta-lactam ring in their molecular structures. This includes penicillin derivatives (penams), cephalosporins (cephems), monobactams, and carbapenems. Most beta-lactam antibiotics work by inhibiting cell wall biosynthesis in the bacterial organism, and they are the most widely used group of antibiotics. Tetracycline is also a broad spectrum antibiotic frequently employed in dairy cow production.

This kit is manufactured to the international quality standard ISO 9001:2008.

AuroFlow™ PR1ME™ BT Combo MRL Assay Specs

The AuroFlow PR1ME BT Combo MRL Assay has the capacity for 50 (Cat # 1134-01) or 100 (Cat # 1134-02) determinations. The shelf life is 12 months when the kit is properly stored.

The positive and negative control vials contain lyophilized milk samples, and must be reconstituted with 1 mL of distilled water before use. The reconstituted raw milk controls are stable for 24 hours at 4°C.



AuroFlow™ PR1ME™ BT Combo Strip Test Kit Protocol


AuroFlow™ PR1ME™ BT Combo MRL Assay Visual Interpretation



The above diagram is for visual interpretation only. If using the QuickSTAR™ Strip Reader, refer to reader instructions provided with the unit.If any test line is less intense than the control line, the sample is positive for that antibiotic.

Invalid Results 

The test is invalid if no colored line appears in the control line area, even if a colored line appears in the test line area. Re-test milk sample if broken lines are observed. Colored lines which appear after 10 minutes are not diagnostic and should be ignored. If the sample does not flow up the dipstick, the milk sample may be clotted, invalidating the test result. To avoid milk sample clotting, always use fresh raw milk.