MaxSignal® Histamine Enzymatic Assay Kit

 

Catalog# Product Name Quantity US List Price
1032-09 MaxSignal® Histamine Enzymatic Assay Kit 1 x 96 wells Inquire

This kit is available for purchase by email, fax or phone only. Please email your order to Bioo.Sales@PerkinElmer.com, fax it to 1-512-707-8122 or call 512-707-8993.

MaxSignal Histamine Enzymatic Assay Kit

    • AOAC approved histamine testing for seafood
    • Simple - Add sample and master mix to plate
    • Sensitive histamine detection - 4 ppm detection limit
    • Faster results – As little as 15 minutes from sample to results
    • Saves money - No expensive instrumentation required
    • High recovery rates

     

 

Sensitive Histamine Detection in Fish, Seafood and Cheese

The MaxSignal® Histamine Enzymatic Assay Kit is a colorimetric enzymatic assay for the determination of histamine content in fresh fish and seafood in less than 15 minutes. The kit offers efficient and solvent free sample preparation, and recovery rates consistent with the AOAC’s rigorous performance standards. This product has been issued the AOAC PTM certificate number 051701, certifying that the method’s performance was reviewed by AOAC Research Institute and it was found to perform according to the manufacturer’s specifications. The AOAC Performance Tested Methods (PTM) program is the most widely recognized, worldwide method certification program for the reliability and performance of proprietary methods. Methods certified as Performance Tested have been found by the AOAC to perform according to manufacturer-documented claims, and are trusted by buyers for use by regulatory bodies and in the global marketplace.

Histamine is a contaminant found in improperly handled seafood. High levels of histamine in seafood can cause scombroid poisoning. Quality tuna has histamine levels below 50 ppm. The amount of histamine can be determined using ELISA or LC-MS. However these traditional methods require expensive instrumentation and time-consuming sample preparation procedures. In contrast, the MaxSignal® Histamine Enzymatic Assay Kit offers a rapid and inexpensive method allowing high throughput.


Kit Specs

This kit is AOAC approved with the certificate number AOAC #051701 and is manufactured to the international quality standard ISO 9001:2008.

PLATE: 1 x 96 well (Removable 8 wells x 12 strips) 
MEASUREMENT: Microtiter plate reader (450 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate 
STANDARDS: 0 / 1 / 2 / 4 / 8 / 12 ppm
INCUBATION TIME: 5 minutes 
SHELF LIFE & STORAGE: 12 months when properly stored


Table 1. Sample homogeneity of all the native matrices was confirmed by AOAC and enzymatic method and illustrated in the below table. The evaluation of spiked matrices indicates an overall recovery of 90 - 102%.

 

Table 2. AOAC 977.13 OMA method was used for establishing background histamine content in frozen/fresh tuna, canned tuna, pouched tuna and mahi-mahi samples used in the study.