High sensitivity and accuracy
- A robust, rapid 10 minutes enzyme-based assay
- High reproducibility
- Low sample requirement – only 5 µL of serum is needed
- Does not require expensive instrumentation
The MaxDiscovery™ Glucose Assay Kit is a plate-based colorimetric enzymatic assay for the determination of glucose in serum samples.
MaxDiscovery Glucose Assay Kit is a simple, direct and automation-compatible method for measuring glucose levels in serum samples. This kit uses a coupled enzymatic reaction scheme: Glucose is first oxidized by glucose oxidase enzyme to form gluconic acid and hydrogen peroxide. In the second step, the hydrogen peroxide reacts with a chromogenic dye using peroxidase to form a visibly-colored (red) dye product. The amount of dye product, measured by the sample absorbance at 520 nm, is proportional to the concentration of glucose in the sample. The kit comes with a control solution containing a glucose standard (100 mg/dL), which is used to calibrate the assay.
Glucose is the most prominent carbohydrate found in blood. The metabolism of glucose is the chief source of cellular energy throughout the body. The determination of serum glucose is an important marker for a number of disease states. Elevated levels of glucose are strongly associated with diabetes and other diseases such as liver or thyroid diseases, renal failure, and pancreatitis while low glucose levels are linked to insulin-induced hypoglycemia and neoplasms.
The MaxDiscovery Glucose Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). Store the kit (except for the microplate) at 4°C. The shelf life of the kit is 6 months when the kit is properly stored. Once the Reagent Mix is reconstituted with water, the shelf-life is 2 months when properly stored.