High sensitivity and low detection limit (5 mg/dL)
- A rapid (10 minutes) and robust enzyme-based assay which does not require expensive instrumentation
- High reproducibility
- Only requires 5 µL of serum
The MaxDiscovery™ Triglycerides Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of triglycerides in serum samples. Triglycerides are a prominent class of lipids found in blood. Triglycerides are used to form cell membranes and hormones, and play important roles in signal transduction pathways. The determination of serum triglycerides is an important marker for the diagnosis of lipemias. Elevated levels of triglycerides are strongly associated with vascular diseases such as stroke and atherosclerosis.
MaxDiscovery Triglycerides Enzymatic Assay Kit is a simple, direct and automation-compatible method for measuring triglyceride levels in serum samples. This kit uses a coupled enzymatic reaction scheme: triglyceride esters are first converted to glycerol and fatty acids. Next, glycerol is converted using ATP to glycerol-phosphate. Lastly, the glycerol-phosphate is enzymatically oxidized to form hydrogen peroxide, which is visibly detected (at 520 nm) by peroxidase using a chromogenic dye. The absorption measured at 520 nm, is proportional to the concentration of triglycerides in the sample. The kit also comes with a control solution containing a reference standard (200 mg/dL), which can be used to calibrate the assay.
This kit provides direct determination of triglycerides in serum, plasma, and other fluid samples. In addition, the kit can be used to analyze the pharmacological effects of drugs on triglycerides metabolism.
Evans, J.F. et al. (2012) The lipocalin-type prostaglandin D2 synthase knock-out (L-PGDS KO) mouse model of insulin resistance and obesity demonstrates early hypothalamic-pituitary-adrenal (HPA) axis hyperactivity. Journal of Endocrinology. doi: 10.1530/JOE-12-0275
The MaxDiscovery Triglycerides Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). Store the kit (except for the microplate) at 4°C. The shelf life of the kit is 6 months when the kit is properly stored. Once the Reagent Mix is reconstituted with water, the shelf-life is 2 months when properly stored.