Rapid and simple method
- Minimal sample prep
- Highly accurate and reproducible
- High sensitivity (3 U/L detection limit)
The MaxDiscovery™ Alkaline Phosphatase (AP) CE Assay Kit is a plate-based, colorimetric-endpoint assay for the determination of alkaline phosphatase enzyme in serum from rodents or other mammals.
Alkaline phosphatase (AP) is a ubiquitously-expressed intracellular enzyme which catalyzes the hydrolysis of organic phosphoesters. AP is a clinically important protein marker in serum because its level changes in response to a number of health-related states. For example, changes in AP serum levels are often caused by liver and liver and bone diseases. Also, the presence of elevated levels of the enzyme in serum after administration of drugs and experimental therapeutic agents is associated with organ toxicity. Therefore, monitoring serum levels of AP enzyme has become a routine method to monitor drug toxicity.
MaxDiscovery Alkaline Phosphatase CE Assay Kit uses an enzymatic assay to determine the amount of alkaline phosphatase in rodent serum and other liquid samples. The kit enables biomedical researchers to determine alkaline phosphatase levels in liquid samples such as serum. The MaxDiscovery™ Alkaline Phosphatase CE Assay Kit contains sufficient materials to test 42 samples in duplicate.
The assay utilizes a simple colorimetric (visible) enzymatic assay to specifically detect AP in fluids. The MaxDiscovery Alkaline Phosphatase CE Assay Kit provides accurate, proven results even in complex liquid mixtures. The kit is designed to be used with a microplate reader. The kit contains a thymolphthalein (TP) standard to construct a linear calibration curve and verify assay performance.
The MaxDiscovery Alkaline Phosphatase (AP) CE Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for serially-diluted standards). Upon receipt of the kit, place the dilution buffer at -20°C. Store the remainder of the kit at 4 °C. The shelf life is 6 months when the kit is properly store.