High sensitivity with low detection limit (20 U/L)
- Rapid – results in 5 minutes
- Robust kinetic assay
- Does not require expensive instrumentation
- Highly reproducible results
- Only requires 10 µL of serum
- Measure absorbance in UV spectrum (340 nm)
The MaxDiscovery™ Aspartate Transaminase (AST) Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of the aspartate transaminase enzyme in 10 µL of serum from rodents or other mammals. Aspartate transaminase (AST) also known as aspartate aminotransferase or (sGOT) is a metabolic enzyme expressed primarily in the liver. Elevation of AST levels is an indication of liver damage and has been associated with liver injury. AST levels are monitored routinely in patients with liver diseases. AST is also a very useful tool for preclinical investigation of experimental drug formulations and AST levels are commonly used to monitor and attenuate the hepatotoxic effects of experimental drugs in rodents.
Fang X, Wang R, Ma J, Ding Y, Shang W, et al. (2012) Ameliorated ConA-Induced Hepatitis in the Absence of PKC-theta. PLoS ONE 7(2): e31174. doi:10.1371/journal.pone.0031174
Kühnel, F. et al. (29 December 2009) Targeting of p53-Transcriptional Dysfunction by Conditionally Replicating Adenovirus Is Not Limited by p53-Homologues. Mol. Ther. http://www.nature.com/mt/journal/vaop/ncurrent/full/mt2009298a.html
Patel R, Baker SS, Liu W, Desai S, Alkhouri R, et al. (2012) Effect of Dietary Advanced Glycation End Products on Mouse Liver. PLoS ONE 7(4): e35143. doi:10.1371/journal.pone.0035143
Wu, S.Y. et al. (12 August 2010) Systemic delivery of E6/7 siRNA using novel lipidic particles and its application with cisplatin in cervical cancer mouse models. Gene Therapy. http://www.nature.com/gt/journal/vaop/ncurrent/full/gt2010113a.html
Rink, C. et al. (2011) Oxygen-Inducible Glutamate Oxaloaceate Transaminase as Protective Switch Transforming Neurotoxic Glutamate to Metabolic Fuel During Acute Ischemic Stroke. Antioxid Redox Sign., 14: 1777 – 1785.
Venkatesh, M., et al. (July, 2011) In Vivo and In Vitro Characterization of a First-in-Class Novel Azole Analog That Targets Pregnane X Receptor Activation. Molecular Pharmacology 80(1): 124-135.
The MaxDiscovery Aspartate Transaminase (AST) Enzymatic Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. Store the kit at 4°C. The shelf life of the kit is 6 months, after receipt, when the kit is properly stored.