High sensitivity with low detection limit (20 U/L)
- Rapid – results in 5 minutes
- Does not require expensive instrumentation
- Highly reproducible results
- Requires only 5 µL of serum
- Measure absorbance in UV spectrum (340 nm)
The MaxDiscovery™ Creatine Kinase (CK) Enzymatic Assay Kit is a colorimetric assay for the determination of creatine kinase in liquid samples.
The MaxDiscovery Creatine Kinase (CK) Enzymatic Assay Kit measures the concentration of creatine kinase using the creatine kinase enzyme, which converts ADP to ATP. The ATP produced by the creatine kinase is then detected by a coupled enzymatic reaction in which the ATP is first used to produce 6-glucose phosphate which is then used by a third enzyme to produce NADH from NAD+, which is monitored by the absorbance change at 340 nm. The kit also contains NADH standards to construct a linear calibration curve and verify assay performance.
FORMAT: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: Microtiter plate reader (340 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards).
SHELF LIFE: 6 months when stored under optimal conditions
SAMPLE SIZE: 5 μL/well
SAMPLE TYPE: Serum