Rapid, highly sensitive assay with low detection limit (10 U/L)
- Convenient, visible, colorimetric, end-point assay
- Does not require expensive instrumentation
- High reproducibility
- Requires only 5 µL of serum
The MaxDiscovery™ Lactate Dehydrogenase (LDH) Color Endpoint Assay Kit is a novel, plate-based colorimetric enzymatic assay for the determination of the lactate dehydrogenase enzyme in mammalian serum samples. L-Lactate Dehydrogenase (LDH) is a ubiquitously-expressed intracellular enzyme which catalyzes the reversible oxidation of lactate to pyruvate. LDH is one of the most clinically important protein markers in serum because its level changes in response to a number of health-related states.
For example, elevated LDH serum levels are often caused by heart, liver and kidney disease as well as in numerous types of cancer. Also, the presence of elevated levels of the enzyme in serum after administration of drugs and experimental therapeutic agents is associated with organ toxicity. In addition, this enzyme can be used to detect cytotoxicity and cell number of in vitro cell culture systems. Therefore, monitoring serum levels of LDH enzyme has become a routine and fundamental means to monitor organ toxicity.
The MaxDiscovery™ Lactate Dehydrogenase (LDH) Color Endpoint Assay Kit uses a unique colored reaction for specific visible detection of LDH activity. In this method, L-lactate and NAD+ are first converted by the LDH enzyme to pyruvate and NADH. The pyruvate then reacts with a hydrazine reagent to form a colored product. The concentration of LDH in each sample is determined by measuring the absorbance at 510 nm. Dilutions of the Pyruvate Control, included in the kit, can be used to construct a standard curve to calibrate the assay and confirm assay linearity.
Maslanik, T. et al. (2012) The inflammasome and Danger Associated Molecular Patterns (DAMPs) are implicated in cytokine and chemokine responses following stressor exposure. Department of Integrative Physiology and the Center for Neuroscience, University of Colorado at Boulder. http://dx.doi.org/10.1016/j.bbi.2012.10.014
The MaxDiscovery™ Lactate Dehydrogenase (LDH) Color Endpoint Assay Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (using 12 wells for standards). The kit also contains enough material to construct four standard curves. Store the kit at 4°C. The shelf life of the kit is 12 months when properly stored.