NEXTflex™ 16S V4 Amplicon-Seq Kit

Validated amplicon-seq library prep for bacterial metagenomics studies

Catalog# Product Name Quantity US List Price
4201-01 NEXTflex™ 16S V4 Amplicon-Seq Kit (with 4 barcodes) 16 rxns $330
4201-02 NEXTflex™ 16S V4 Amplicon-Seq Kit (with 12 barcodes) 48 rxns $773
4201-03 NEXTflex™ 16S V4 Amplicon-Seq Kit (with 24 barcodes) 96 rxns $1,468
4201-04 NEXTflex™ 16S V4 Amplicon-Seq Kit (with 48 barcodes) 192 rxns $2,789
4201-05 NEXTflex™ 16S V4 Amplicon-Seq Kit (with 96 barcodes) 384 rxns $5,298
4201-07 NEXTflex™ 16S V4 Amplicon-Seq Kit (with 288 barcodes) 1,152 rxns $13,648

 

 

 

  • Fast - 2 hour library prep kit
  • Easy - Two step protocol
  • Low input - Only 50 ng of input required
  • Maximized recovery rates
  • Flexible barcode options– Kits containing 4, 12, 24, 48, 96 or 288 unique barcodes
  • Automation-friendly workflow is compatible with liquid handlers
  • Functionally validated with Illumina sequencing platforms 

 

 

 

The NEXTflex™ 16S V4 Amplicon-Seq Kit is a validated amplicon-seq library prep kit developed to simplify bacterial metagenomics studies using Illumina® HiSeq and MiSeq platforms.


Fast, Two-Step Library Prep Protocol

The NEXTflex™ 16S V4 Amplicon-Seq Kit offers a fast two step, gel-free protocol that reduces library prep time to 2 hours and allows for complete automation of bacterial metagenomics studies. There are two main steps involved in the NEXTflex 16S V4 Amplicon-Seq Kit protocol: amplification and cleanup. Primers included in the NEXTflex 16S V4 Amplicon-Seq Kit are designed to hybridize to the fourth hypervariable (V4) domain of microbial 16S ribosomal RNA (rRNA) genes, allowing for amplification and sequencing of the variable regions. The single cleanup step ensures maximum recovery of amplicons for downstream sequencing.

Users interested in different degrees of multiplexing should contact nextgen@biooscientific.com.

The NEXTflex 16S V4 Amplicon-Seq Kit utilizes an excellent phylogenetic marker, allowing for studies of entire microbial communities or individual taxa, and is ideal for monitoring population fluctuations.

 

Kit Specs

The NEXTflex 16S V4 Amplicon-Seq Kit contains enough material to prepare 16, 48, 96, 192, 384, or 1,152 samples of bacterial genomic DNA for Illumina® compatible sequencing. The shelf life of all reagents is 12 months when stored properly. All components can be safely stored at -20°C. This kit is shipped on dry ice.

NEXTflex™ 16S V4 Amplicon-Seq Protocol