NEXTflex™ ChIP-Seq Kit

Library preparation for Illumina sequencing platforms using ChIP or genomic DNA

Catalog# Product Name Quantity US List Price
5143-01 NEXTflex™ ChIP-Seq Kit 8 rxns $260
5143-02 NEXTflex™ ChIP-Seq Kit 48 rxns $1,573
514120 NEXTflex™ ChIP-Seq Barcodes - 6 48 rxns $213
514121 NEXTflex™ ChIP-Seq Barcodes - 12 96 rxns $421
514122 NEXTflex™ ChIP-Seq Barcodes - 24 192 rxns $833
514123 NEXTflex™ ChIP-Seq Barcodes - 48 384 rxns $1,639
514124 NEXTflex™ ChIP-Seq Barcodes - 96 768 rxns $3,169

 

  • Ultra low DNA input requirement of 1 ng
  • For use with ChIP or genomic DNA samples
  • Optimized for low DNA input with NanoQ™ enzymes and buffers
  • Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
  • 96 barcoded adapters for multiplexing contain embedded index sequence
  • Fast bead-based cleanup protocol shaves hours off of library prep time
  • Automation-friendly workflow is compatible with liquid handlers
  • Flexible barcode options – Kits containing 6, 12, 24, 48, and 96 unique barcodes
  • Compatible with Illumina sequencing platforms

 

 

The NEXTflex™ ChIP-Seq Kit contains specially designed NanoQ™ enzymes and buffers which allow for ultra low DNA input requirements of only 1 ng for sample preparation of chromatin immunoprecipitated or regular genomic DNA for next generation sequencing. The NEXTflex ChIP-Seq Kit also features Bioo Scientific’s proprietary Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. With these improvements to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters, and can expect to see better binding efficiencies. 


Simple Automation Friendly Workflow 

The NEXTflex ChIP-Seq Kit simplifies the library prep workflow by using master mixed reagents and a gel-free protocol using magnetic bead based cleanup. 

 

Improved ChIP-Seq Quantitation 

Do you need to improve the quantitation of your ChIP-Seq experiments? Molecular Indexes™ can be incorporated into your library prep, thus allowing identical DNA molecules in the starting material to be distinguished from PCR duplicates, allowing accurate quantitative analysis of ChIP-seq data.  In addition, molecular indexing is an ideal technology for studying SNPs, as polymerase errors can be easily distinguished from true polymorphisms. To learn more about incorporating Molecular Indexes into ChIP-Seq work flow visit the NEXTflex qRNA-Seq Kit page or read our blog post, Improved ChIP-Seq Quantitation with Molecular Indexing™.


Unparalleled Multiplexing Capabilities 

 Using the NEXTflex™ ChIP-Seq Barcodes, the user can index 6, 12, 24, 48, or even 96 samples at once providing unparalleled ChIP sequencing capacity.

For larger volume requirements, customization and bulk packaging is available. For increased flexibility, individual reagents (non-master mixed) are also available. Please contact nextgen@biooscientific.com for further information. 

 

Selected References: 

Xu, Z., Chen, H., Ling, J., Yu, D., Struffi, P., and Small, S. (2014) Impacts of the ubiquitous factor Zelda on Bicoid-dependent DNA binding and transcription in Drosophila. Genes & Dev. 28: 608 - 621.

Jiang, L. et al. (2014) ZBED6 Modulates the Transcription of Myogenic Genes in Mouse Myoblast Cells. PLoS ONE 9(4): e94187. doi: 10.1371/journal.pone.0094187 

Clop A, Bertoni A, Spain SL, Simpson MA, Pullabhatla V, et al. (2013) An In-Depth Characterization of the Major Psoriasis Susceptibility Locus Identifies Candidate Susceptibility Alleles within an HLA-C Enhancer Element. PLoS ONE 8(8): e71690. doi:10.1371/journal.pone.0071690

Cvejic A, Haer-Wigman L, Stephens JC et al. (2013) SMIM1 underlies the Vel blood group and influences red blood cell traits. Nature Genetics. doi:10.1038/ng.2603 

Lam MTY, Cho H, Lesch HP et al. (2013) Rev-Erbs repress macrophage gene expression by inhibiting enhancer-directed transcription. Nature. doi:10.1038/nature12209 

Rossetto CC, Tarrant-Elorza M, Pari GS (2013) Cis and Trans Acting Factors Involved in Human Cytomegalovirus Experimental and Natural Latent Infection of CD14 (+) Monocytes and CD34 (+) Cells. PLoS Pathog 9(5): e1003366. doi:10.1371/journal.ppat.1003366 

Thakur J. and Sanyal K. (Feb 2013) Efficient neocentromere formation is suppressed by gene conversion to maintain centromere function at native physical chromosomal loci in Candida albicans. Genome Research. doi: 10.1101/gr.141614.11

Watson E., MacNeil L.T. et al. (2013) Integration of Metabolic and Gene Regulatory Networks Modulates the C. elegans Dietary Response. Cell. 

 

Kit Specs

The NEXTflex ChIP-Seq Kit contains enough material to prepare 8 or 48 ChIP or genomic DNA samples for Illumina® compatible sequencing. The shelf life of all reagents is 12 months when stored properly. This kit is shipped on dry ice.

NEXTflex™ ChIP-Seq Protocol

 

NEXTflex ChIP-Seq Library Prep Protocol for Illumina Sequencing