- Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
- Completely gel-free library prep protocol available
- Flexible barcode options– Kits containing 6, 12, 24, 48, or 96 or 192 barcodes
- Simplified workflow with master mixes that reduce hands-on time
- Automation-friendly workflow is compatible with liquid handlers
- Barcoded adapters for multiplexing contain embedded index sequence
- Bead-based cleanup protocol
- Cost-effective library prep solution
- Functionally validated with Illumina sequencing platforms
The NEXTflex™ DNA Sequencing Kit is designed to prepare single, paired-end, and multiplexed genomic DNA libraries for sequencing using Illumina® platforms. The enhanced NEXTflex™ kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. The NEXTflex DNA Sequencing Kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. With these changes to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters, and can expect to see better binding efficiencies.
Flexible Multiplexing Options
The NEXTflex™ Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. The NEXTflex DNA Sequening Kit is designed to be used with the NEXTflex™ DNA Barcodes and NEXTflex-96™ DNA Barcodes. These barcodes are available in sets of 6, 12, 24, 48 and 96 unique indexed adapters. NEXTflex Dual-Indexed DNA Barcodes are now available that allow for the multiplexing of up to 192 samples.
Completely Gel-Free, DNA-Seq Library Prep Protocol
The NEXTflex DNA Sequencing Kit now includes an optional protocol which allows you to completely eliminate the gel purification step. The alternative bead-based protocol reduces the required sample prep time by 2 hours and allows for complete automation of DNA-seq library prep.
Download a copy of our Application Note, Tools for Enhanced Sequence Diversity and Reducing Bias in DNA-seq Library Preparation, which was recently published on Nature Methods.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility, individual reagents (non-master mixed) are also available. Please contact firstname.lastname@example.org for further information.
Duguma D, Rugman-Jones P, Kaufman MG, Hall MW, Neufeld JD, et al. (2013) Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance. PLoS ONE 8(8): e72522. doi:10.1371/journal.pone.0072522
Evrony, Cai, et al. (2012) Single-Neuron Sequencing Analysis of L1 Retrotransposition and Somatic Mutation in the Human Brain. Cell. Vol. 151, Issue 3, pp. 483-496.
Herron MD, Doebeli M (2013) Parallel Evolutionary Dynamics of Adaptive Diversification in Escherichia coli. PLoS Biol 11(2): e1001490. doi:10.1371/journal.pbio.1001490
Obholzer N. et al. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing. doi: 10.1242/dev.083931
Tech Tips - When to Size Select
Size selection is a critical issue in NGS library preparation. Bioo Scientific’s blog post, Tech Tips – When to Size Select, addresses a number of factors to consider before determining a size selection strategy.
The NEXTflex DNA Sequencing Kits contain the reagents to prepare 8 or 48 genomic DNA samples for Illumina®-compatible DNA-seq next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.