NEXTflex™ DNA Sequencing Kit

Library prep for sequencing on Illumina platforms

Catalog# Product Name Quantity US List Price
5140-01 NEXTflex™ DNA Sequencing Kit 8 rxns $328
5140-02 NEXTflex™ DNA Sequencing Kit 48 rxns $1,573
514101 NEXTflex™ DNA Barcodes - 6 48 rxns $426
514102 NEXTflex™ DNA Barcodes - 12 96 rxns $841
514103 NEXTflex™ DNA Barcodes - 24 192 rxns $1,667
514104 NEXTflex™ DNA Barcodes - 48 384 rxns $3,278
514105 NEXTflex-96™ DNA Barcodes - 96 (in 96-well plate) 768 rxns $6,338
514106 NEXTflex-96™ DNA Barcodes - 96 (in tubes) 768 rxns $6,338

  • Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
  • Completely gel-free library prep protocol available
  • Flexible barcode options– Kits containing 6, 12, 24, 48, or 96 or 192 barcodes
  • Simplified workflow with master mixes that reduce hands-on time
  • Automation-friendly workflow is compatible with liquid handlers
  • Barcoded adapters for multiplexing contain embedded index sequence
  • Bead-based cleanup protocol
  • Cost-effective library prep solution
  • Functionally validated with Illumina sequencing platforms



The NEXTflex™ DNA Sequencing Kit is designed to prepare single, paired-end, and multiplexed genomic DNA libraries for sequencing using Illumina® platforms. The enhanced NEXTflex™ kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. The NEXTflex DNA Sequencing Kit features Enhanced Adapter Ligation Technology, resulting in library preps with a larger number of unique sequencing reads. With these changes to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters, and can expect to see better binding efficiencies.

Flexible Multiplexing Options

The NEXTflex™ Adapters are long, annealed adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes can be used with single, paired-end and multiplex reads. The NEXTflex DNA Sequening Kit is designed to be used with the NEXTflex™ DNA Barcodes and NEXTflex-96™ DNA Barcodes. These barcodes are available in sets of 6, 12, 24, 48 and 96 unique indexed adapters. NEXTflex Dual-Indexed DNA Barcodes are now available that allow for the multiplexing of up to 192 samples.


Completely Gel-Free, DNA-Seq Library Prep Protocol 

The NEXTflex DNA Sequencing Kit now includes an optional protocol which allows you to completely eliminate the gel purification step. The alternative bead-based protocol reduces the required sample prep time by 2 hours and allows for complete automation of DNA-seq library prep.

Download a copy of our Application Note, Tools for Enhanced Sequence Diversity and Reducing Bias in DNA-seq Library Preparation, which was recently published on Nature Methods. 

For larger volume requirements, customization and bulk packaging is available. For increased flexibility, individual reagents (non-master mixed) are also available. Please contact for further information. 


Selected References: 

Duguma D, Rugman-Jones P, Kaufman MG, Hall MW, Neufeld JD, et al. (2013) Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance. PLoS ONE 8(8): e72522. doi:10.1371/journal.pone.0072522 

Evrony, Cai, et al. (2012) Single-Neuron Sequencing Analysis of L1 Retrotransposition and Somatic Mutation in the Human Brain. Cell. Vol. 151, Issue 3, pp. 483-496. 

Herron MD, Doebeli M (2013) Parallel Evolutionary Dynamics of Adaptive Diversification in Escherichia coli. PLoS Biol 11(2): e1001490. doi:10.1371/journal.pbio.1001490 

Obholzer N. et al. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing. doi: 10.1242/dev.083931

Tech Tips - When to Size Select 

Size selection is a critical issue in NGS library preparation. Bioo Scientific’s blog post, Tech Tips – When to Size Select, addresses a number of factors to consider before determining a size selection strategy.


Kit Specs

The NEXTflex DNA Sequencing Kits contain the reagents to prepare 8 or 48 genomic DNA samples for Illumina®-compatible DNA-seq next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. This kit is shipped on dry ice.

DNA-Seq Protocol



Summary of Sequence Data between NEXTflex™ and Competitor X's Libraries