- Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
- Input - 10 - 100 ng of mRNA or rRNA depleted total RNA
- Up to 96 barcodes available for multiplexing contain embedded index sequence
- Automation-friendly workflow is compatible with liquid handlers
- Functionally validated with Illumina sequencing platforms
The patent pending NEXTflex™ RNA-Seq Kits provide an easy and flexible solution for generating single, paired-end and multiplexed libraries from mRNA or rRNA depleted total RNA. Using the NEXTflex™ RNA-Seq library prep protocol, mRNA or rRNA depleted RNA is fragmented using a cationic buffer. Fragmented RNA undergoes first and second strand synthesis, followed by end-repair, adenylation, ligation and PCR.
We have recently launched the NEXTflex™ Rapid RNA-Seq Kit which incorporates a faster, streamlined protocol for RNAseq library prep and also includes the thermostable NEXTflex™ Rapid Reverse Transcriptase which is optimized for NGS applications.
This kit was designed to be used in conjunction with the NEXTflex™ RNA-Seq or NEXTflex-96™ RNA-Seq Barcodes for multiplexing. The availability of up to 96 unique adapter barcodes makes this the most high-throughput kit available for RNA-Seq.
Magnetic Beads for mRNA Purification
The NEXTflex™ Poly(A) Beads now provide a convenient method for batch purification of pure, intact mRNA upstream NEXTflex RNA-Seq library preparation. NEXTflex Poly(A) Beads beads use oligo(dT) primer to isolate polyadenylated messenger RNAs from 1 µg – 10 µg of previously isolated total RNA.
The NEXTflex RNA-Seq Kit contains enough material to prepare 8 or 48 RNA samples for Illumina® compatible sequencing. The shelf life of all reagents is 6 months when stored properly. All components can safely be stored at -20°C.
NEXTflex™ RNA-Seq Protocol
Relative Levels of ERCC Control RNAs in RNA-Seq Libraries
Made from NEXTflex Poly(A) Bead Selected and Total RNA Correlation between expected and observed values for percentage of reads mapping to 94 ERCC control RNAs in RNA-Seq library made using 20 µL of NEXTflex Poly(A) Beads.
Relative Levels of Different Categories of Endogenous Transcripts Detected in RNA-Seq Libraries made from NEXTflex Poly(A) Bead Selected and Total RNA
Percentage of reads mapping to rRNA regions and regions of interest with and without Poly(A) bead selection.