- Up to 48 barcodes available for multiplexing
- Utilizes AIR™ Ligase, a highly efficient truncated T4 RNA Ligase, for greater sequencing depth
- Simplified workflow reduces hands-on time
- Flexible barcode options
- Barcoded PCR Primers for multiplexing contain embedded index sequence
- Automation-friendly workflow is compatible with liquid handlers
- Functionally validated with the Illumina sequencing platforms
The patent pending NEXTflex™ Small RNA Sequencing Kits provide an easy, flexible, cost-effective solution for generating single and multiplexed libraries from small RNA using Illumina sequencing platforms. This kit contains a specialized 3’ adenylated adapter that specifically ligates onto microRNAs and other small RNAs containing a 3’ hydroxyl group. After adapter ligation and reverse transcription, libraries are amplified using a barcoded primer, allowing up to 48 samples to be multiplexed and sequence variations interrogated. The NEXTflex™ Small RNA Sequencing Kit incorporates AIR™ Ligase, an enhanced, truncated T4 RNA Ligase, which increases the efficiency with which small RNAs are tagged with adapter, giving greater sequence depth. The innovative NEXTflex adapter ligation technology that is incorporated into the NEXTflex Small RNA Sequencing Kit greatly reduces the adapter dimer band giving better quality and a higher number of sequence reads with less contamination from adapter dimer. Using a specialized RT primer and buffer strategy, 3’ and 5’ adapter dimers will no longer impede your research by taking up valuable sequencing reads.
The NEXTflex™ Small RNA Sequencing Kit on its own contains all the material to prepare 24 or 48 singleplex libraries. In combination with the NEXTflex™ Small RNA Barcode Primer Kits, 48 samples can be profiled and studied simultaneously. The NEXTflex Small RNA Barcode Primer Kits contain 8 reactions each of 12 unique barcodes for mulltiplexing. In addition to small RNA sequencing, the kit is compatible with RIP-Seq and CLIP-Seq protocols.
For larger volume requirements, customization and bulk packaging is available. For increased flexibility individual reagents (non-master mixed) are also available. Please contact email@example.com for further information.
Reducing Bias in Small RNA-Seq Library Prep:
Read our blog post, Reducing Bias in Small RNA-Seq Library Prep, describing new technology available to reduce bias introduced by ligation in small RNA sequencing library preparation.
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The NEXTflex™ Small RNA-Seq Kits contain the reagents to prepare 24 or 48 small RNA samples for Illumina®-compatible next-generation sequencing. The shelf life of all reagents is 12 months when stored properly at -20°C. Ships on dry ice.