- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 100 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Rat IFN-γ ELISA Test Kit is designed for quantitative determination of the concentration of rat IFN-γ in serum, plasma, and cell culture supernatant.
Rat Interferon gamma (IFN-γ), also known as immune interferon, is a ~15 kDa protein of 137 amino acids (aa) after cleavage of a 19 aa signal peptide. It is presumed that Rat IFN-γ is a noncovalent homodimer. IFN-γ is the only member of the Type II class of interferons. It is produced by activated T, B and NK cells and is an important regulatory cytokine. IFN-γ plays a key role in host defense as it has antiviral, immunoregulatory, and anti-tumor properties. IFN-γ induces the production of cytokines and up-regulates the expression of various membrane proteins including class I and II MHC antigens, Fc receptors, leukocyte adhesion molecules, and B7 antigen. In the mature segment, rat IFN-γ is 87% identical to mouse IFN-γ and 40% identical to human IFN-γ.
Format: 96-well strip plate
Assay range: 100-6400 pg/mL
Sensitivity: 100 pg/mL
Total Assay Incubation Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm