- Complete, ready-to-use kit
- Cost effective
- Quantitative determination of protein concentration
- Sensitive - detect as little as 60 pg/mL
- Reliable - thoroughly tested gene-specific kit
- Colorimetric detection
The MaxDiscovery™ Rat IL-6 ELISA Test Kit is designed for quantitative determination of the concentration of rat IL-6 in serum, plasma, and cell culture supernatant.
MaxDiscovery™ Rat IL-6 ELISA Test Kit is designed for quantitative determination of the concentration of rat interleukin 6 (IL-6) in serum, plasma, and cell culture supernatant. Rat interleukin 6 (IL-6) contains 187 amino acid residues after cleavage of a signal peptide of 24 amino acids. It is a multifunctional cytokine that plays important roles in host defense, acute phase reactions, immune responses, nerve cell functions and hemotopoiesis. IL-6 is expressed by a variety of normal and transformed lymphoid and nonlymphoid cells. The production of IL-6 is up-regulated by numerous signals such as mitogenic or antigenic stimulation, lipopolysaccharides, calcium ionophores, cytokines and viruses. IL-4, IL-10 and IL-3 inhibit IL-6 expression in monocytes. Elevated serum IL-6 levels have been observed in a number of pathological conditions, including bacterial and viral infections, trauma, autoimmune diseases, inflammations and malignancies. At the protein sequence level, there is approximately 39% identity between rat and human IL-6, and 87% identity between mouse and rat IL-6.
Format: 96-well strip plate
Assay range: 60-3840 pg/mL
Sensitivity: 60 pg/mL
Total Assay Incubation Time: 3 hours and 45 minutes
Sample Size: 100 µL/well
Sample types: Serum, Plasma and Cell Culture Supernatant
Wavelength for plate reading: 450 nm