MaxDiscovery™ Rat TNF-α ELISA Test Kit

 

Catalog# Product Name Quantity US List Price
2203-01 MaxDiscovery™ Rat TNF-α ELISA Test Kit 1 x 96 wells $475 Buy Now

 

 

  1. Complete, ready-to-use kit
  2. Cost effective
  3. Quantitative determination of protein concentration
  4. Sensitive - detect as little as 20 pg/mL
  5. Reliable - thoroughly tested gene-specific kit
  6. Colorimetric detection 

 

The MaxDiscovery™ Rat TNF-α ELISA Test Kit is designed for quantitative determination of the concentration of rat TNF-α in serum, plasma, and cell culture supernatant. 


Rat tumor necrosis factor-alpha (TNF-α) is primarily a homotrimeric 17 kDa protein, a potent mediator of inflammatory and metabolic functions. Originally known as a highly cytotoxic cytokine for tumor cells, it is produced by lipopolysaccharide stimulated macrophages. When injected into tumor bearing animal, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further, TNF-α has been implied as central mediator in shock induced by gram negative microorganisms. TNF-α functions as a central mediator in many inflammatory and immunological processes. Interestingly, it also induced on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the p55 and p75 TNF-receptors. The receptors differ strongly in their intracellular signaling pathways


Kit Specs

Format: 96-well strip plate 
Assay range: 20-1280 pg/mL 
Sensitivity: 20 pg/mL 
Total Assay Incubation Time: 3 hours and 45 minutes 
Sample Size: 100 µL/well 
Sample types: Serum, Plasma and Cell Culture Supernatant 
Wavelength for plate reading: 450 nm