- Ideal for the quantification of RSV in human samples
- Highly sensitive and reproducible
- Fast and simple protocols
- Suited for automation
- Complete kits including coated plates
The MaxDiscovery™ Respiratory Syncytial Virus (RSV) ELISA Kit is an enzyme immunoassay designed for research use only that quantifies Respiratory Syncytial Virus or RSV from cells or fluid samples. Respiratory Syncytial Virus is a very common virus that leads to mild, cold-like symptoms in adults and older healthy children. Most otherwise healthy people recover from RSV infection in 1 to 2 weeks. However, infection can be severe in some people, such as certain infants, young children, and older adults. In fact, RSV is the most common cause of bronchiolitis (inflammation of the small airways in the lung) and pneumonia in children under 1 year of age in the United States.
Like most ELISA assays, the MaxDiscovery Respiratory Syncytial Virus ELISA Kit relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that is blue when oxidized with hydrogen peroxide (catalyzed by HRP) and has major absorbance peaks at 370 nm and 652 nm. The color changes to yellow with the addition of acid and has a maximum absorbance at 450 nm. The relative amount of Respiratory Syncytial Virus protein is directly proportional to the optical density (OD) reading at 450 nm.
This kit contains materials for the quantitative detection of the RSV protein in human cells or fluid samples.
Note: This kit is designed for research use only. All US orders require that a "Research Use Only" certification form (download below) must be filled out and submitted with your order.
Publications that Cite the Use of the MaxDiscovery™ RSV ELISA
Alonas, E. et. al. (2014) Combining single RNA sensitive probes with subdiffraction-limited and live-cell imaging enables the characterization of virus dynamics in cells. ACS Nano. 8:1, 302-15.
The MaxDiscovery Respiratory Syncytial Virus (RSV) ELISA Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 6 wells for standards, in duplicate). Return any unused microwells to the foil bag and reseal them with the desiccant provided, in the original package. Store the kit at 4°C. The normal shelf life is 6 months from the date of manufacturing when the kit is properly stored.