Duguma from the University of California Riverside along with others from the University of Waterloo and Michigan State University recently characterized bacterial communities associated with late larval instars of the western encephalitis mosquito (Culex tarsalis), the submerged portions of two emergent macrophytes, and the associated water columns to investigate potential differential use of resources by mosquitoes in different wetland habitats. Using the NEXTflex™ DNA-Seq Kit for library prep, they obtained sequence data from 16S rRNA gene hypervariable regions and observed that that the alpha diversity of mosquito gut microbial communities were conserved and did not change across sampling dates and between two distinct plant habitats.
Duguma D, Rugman-Jones P, Kaufman MG, Hall MW, Neufeld JD, et al. (2013) Bacterial Communities Associated with Culex Mosquito Larvae and Two Emergent Aquatic Plants of Bioremediation Importance. PLoS ONE 8(8): e72522. doi:10.1371/journal.pone.0072522.