The Impact of the HIRA Histone Chaperone upon Global Nucleosome Architecture

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The Nicholas Kent Lab at Cardiff University has developed novel next-generation chromatin-seq techniques to analyze chromatin structure and function at a genome-wide level. In this publication, Csenge Gal from the Institute for Cell & Molecular Biosciences at Newcastle University, along with others from the University of Exeter and Nicholas Kent, used the NEXTflex Rapid DNA-Seq Kit to build libraries for chromatin-seq to determine the genome-wide contribution of HIRA in Schizosaccharomyces pombe. HIRA is an evolutionarily conserved histone chaperone that mediates replication-independent nucleosome assembly and is important for a variety of processes. Gal et al. found that HIRA plays an important role in maintaining nucleosome architecture at both euchromatic and heterochromatic loci.

Gal, C. et. al. (2015) The impact of the HIRA histone chaperone upon global nucleosome architecture. Cell Cycle. 14:1, 123-134. doi:10.4161/15384101.2014.967123.

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