Researchers Meng-Han Lin, Daniel F. Jones, and Rachel Fleming determined the NGS analysis of RNA obtained from various fluid samples is a reliable method for body ﬂuid identiﬁcation from the degraded samples frequently encountered at crime scenes, which can be difficult to analyze using the current detection methods, RT-PCR and qPCR.
These researchers have made significant advances in analysis of degraded RNA from forensically relevant fluids, generating significant high quality sequencing output from fresh, two and six week aged samples of oral mucosa/saliva (buccal samples), circulatory blood, menstrual blood and vaginal fluid. Libraries for sequencing were generated using the NEXTflex™ Rapid Directional RNA-Seq Kit, from total RNA which was not subjected to ribosomal RNA depletion. Due to the low concentration and degraded nature of some samples, 13 µL total RNA input was used for library preparation irrespective of concentration.
In these studies, body ﬂuid-speciﬁc markers were detected across different body ﬂuid types and aging intervals and FPKM values for body ﬂuid-speciﬁc markers corresponded to the target body ﬂuid type. The availability of this type of is contextual information may have signiﬁcant implications for the investigation of crimes.
Lin, M.-H., Jones, D. F. and Fleming, R. (2015) Transcriptomic analysis of degraded forensic body fluids. Forensic Science International: Genetics, Volume 17. 35-42. dio: 10.1016/j.fsigen.2015.03.005.