Rapid extraction method for various sample types
- High recovery rates
- High sensitivity - 0.015 ppb
- Quick ELISA assay – less than 2 hours
- High reproducibility
Trimethoprim ELISA with Rapid Extraction Methods
The MaxSignal® Trimethoprim ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Trimethoprim (TMP) in fish, shrimp, feed, beef, chicken, pork, serum and urine.
The MaxSignal Trimethoprim ELISA Test Kit is a competitive colorimetric ELISA assay. The plate wells are coated with Trimethoprim (TMP) antibody. During analysis, sample is added along with the TMP-horseradish peroxidase (TMP-HRP) conjugate. If the TMP residue is present in the sample, it will compete for the TMP antibody, thereby preventing the TMP-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the TMP residue concentration in the sample.
Trimethoprim as a veterinary medicine is used in the prophylaxis and treatment of urinary tract infections. The EEC No. 2377/90 regulation sets the MRL of Trimethoprim at 50 ppb in meat, liver and milk.
This kit is manufactured to the international quality standard ISO 9001:2008.
The MaxSignal® Trimethoprim ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards).
Store the kit at 2-8°C. The shelf life is 12 months when properly stored.