Accurately measure poly(A) tail length and the 3’ untranslated region of any RNA species
- Rapid - poly(A) tail length of multiple RNAs can be analyzed in less than 3 hours
- Simple, patent pending technology that does not require radioactivity
The patent pending technology of the ALL-TAIL™ Kit for extreme 3’ RACE, can be used to investigate the dynamic changes in poly(A) tail length that take place during gene expression, as well as the changes in 3’ untranslated regions caused by differential poly(A) tail addition site selection in development, differentiation and cancer. This kit enables the precise measurement of 3’ poly(A) tail length along with the ability to identify and characterize the sequence the 3’ end of RNA transcripts. It can also be used to detect miRNA molecules using a linker ligation technique followed by PCR.
Bioo Scientific’s ALL-TAIL Kit provides a sensitive, accurate and highly reproducible assay to measure the poly(A) tail length and the 3’ UTR sequence of any RNA species. First, AIR™ Ligase (a highly efficient truncated T4 RNA ligase 2) efficiently joins an adenylated adaptor oligonucleotide to the 3’ end of the total RNAs. Next reverse transcription is performed using a primer specific to the adenylated adaptor to initiate the production of cDNA. Then a primer designed near the 3’ end of the transcript of interest is used along with the primer specific to the adenylated adaptor to perform PCR amplification of the poly(A) tail and a short stretch of the 3’ UTR. This step may be modified to amplify specific miRNA sequences. Finally, the PCR products are separated on an acrylamide gel and visualized using either SYBR Gold Nucleic Acid Stain or ethidium bromide.
Read the ATP-independent Linker Ligation Poly(A) Tail assay (ALL-TAIL) poster.
Publications Citing the Use of the ALL-TAIL Kit
Huang, M. et al (Sept, 2011) Determination of a phosphorylation site in Nipah virus nucleoprotein and its involvement in virus transcription. J Gen Vir 92(9) 2133 - 2141.
Liu, Y., et al. (2011) Erythropoietin increases expression and function of transient receptor potential canonical 5 channels. Hypertension 58:2, 317-24.
Knüsel, S, Roditi, I. (2013) Insights into the regulation of GPEET procyclin during differentiation from early to late procyclic forms of Trypanosoma brucei. Mol. Biochem. Parasitol. 191(2) 66-74.
Su, L., Li, Z., Bernardy, M., Wiersma, P. A., Cheng, Z. and Xiang, Y. (2015) The complete nucleotide sequence and genome organization of pea streak virus (genus Carlavirus). Archives of Virology. Doi: 10.1007/s00705-015-2467-2.
The ALL-TAIL™ Kit can be used to perform 20 ligation reactions, 30 reverse transcription reactions and 30 PCR reactions. Store the Control A549 Total RNA at -80°C and the rest of the kit at -20°C. Return any unused components to their proper storage temperature. The shelf life of this kit is 6 months.