NGS Barcodes for Illumina Platforms

Barcoded Adapters for NGS Multiplexing

Bioo Scientific offers a complete line of validated NGS barcodes for multiplexing, including indexed NGS adapters for both Illumina and Ion Torrent multiplexing. These barcode sets are designed to give flexibility to researchers and are supplied at concentrations ideal for various applications including DNA-Seq, RNA-Seq, and ChIP-Seq library prep. While they are designed to work with Bioo Scientific’s optimized library prep kits, they are compatible with a broad range of other NGS library prep methods. The sequences of the indexes can be downloaded from the individual barcode product pages.

Instructions for installing NEXTflex Barcode Indices in Illumina Experiment Manager

Read out blog post to learn how to use barcodes with different index lengths in a single run.


Illumina-compatible DNA Barcodes

These barcodes are ideal for DNA library prep applications when starting with 10 ng or more of starting material.

 

NEXTflex-HT™ Barcodes

 - 384 unique single-index adapters for multiplexing Illumina libraries

 

NEXTflex® DNA Barcodes

– Unique adapters available in sets of 6, 12, 24, 48 barcodes

 

NEXTflex-96™ DNA Barcodes

– 96 unique adapters available in sets of 96 barcodes

 

NEXTflex® Dual-Indexed DNA Barcodes

– Dual index adapters for multiplexing up to 192 samples simultaneously

 

NEXTflex® PCR-Free DNA Barcodes

– Unique adapters available in sets of 6, 12, 24, 48 barcodes designed specifically for use with the NEXTflex PCR-Free DNA-Seq Kit


Illumina-compatible ChIP-Seq Barcodes

These barcodes are ideal for ChIP-Seq or genomic DNA library prep applications when starting with less than 10 ng of starting material.

NEXTflex® ChIP-Seq Barcodes – Unique adapters available in sets of 6, 12, 24, 48 barcodes
NEXTflex-96™ ChIP-Seq Barcodes – 96 unique adapters available in sets of 96 barcodes


Illumina-compatible RNA-Seq Barcodes

These barcodes are ideal for RNA-Seq library prep applications.

NEXTflex® RNA-Seq Barcodes - Unique adapters available in sets of 6, 12, 24, 48 barcodes
NEXTflex-96™ RNA-Seq Barcodes – 96 unique adapters available in sets of 96 barcodes


Illumina-compatible Bisulfite-Seq Barcodes

These methylated barcodes are ideal for bisulfite-seq library prep applications.

NEXTflex® Bisulfite-Seq Barcodes – Unique adapters available in sets of 6, 12 and 24 methylated adapters


Ion PGM and Ion Proton-compatible DNA Barcodes

These adapters are ideal for genomic DNA or ChIP-Seq multiplexing applications on Ion Torrent-based platforms.

NEXTflex® DNA Barcodes for Ion Torrent™ Sequencing – Unique barcodes available in sets of 8, 16, 32 and 64 adapters.