NGS Barcodes for Illumina Platforms

Barcoded Adapters for NGS Multiplexing

Bioo Scientific offers a complete line of validated NGS barcodes for multiplexing, including indexed NGS adapters for both Illumina® and Ion Torrent™ multiplexing. These barcode sets are designed to give flexibility to researchers and are supplied at concentrations ideal for various applications including DNA-Seq, RNA-Seq, and ChIP-Seq library prep. While they are designed to work with Bioo Scientific’s optimized library prep kits, they are compatible with a broad range of other NGS library prep methods. The sequences of the indexes can be downloaded from the individual barcode product pages.

Instructions for installing NEXTflex Barcode Indices in Illumina® Experiment Manager

Read out blog post to learn how to use barcodes with different index lengths in a single run.


DNA Barcodes for Illumina® Sequencing

These barcodes are ideal for DNA library prep applications when starting with 10 ng or more of starting material.

 

NEXTflex® Unique Dual Index Barcodes - Unique dual index adapters for multiplexing Illumina® sequencing libraries

NEXTflex-HT™ Barcodes - 384 unique single-index adapters for multiplexing Illumina® libraries

NEXTflex® DNA Barcodes – Unique adapters available in sets of 6, 12, 24, 48 barcodes

NEXTflex-96™ DNA Barcodes – 96 unique adapters available in sets of 96 barcodes

NEXTflex® Dual-Indexed DNA Barcodes – Dual index adapters for multiplexing up to 192 samples simultaneously

NEXTflex® PCR-Free DNA Barcodes– Unique adapters available in sets of 6, 12, 24, 48 barcodes designed specifically for use with the NEXTflex PCR-Free DNA-Seq Kit


ChIP-Seq Barcodes for Illumina® Sequencing

These barcodes are ideal for ChIP-Seq or genomic DNA library prep applications when starting with less than 10 ng of starting material. 

 

NEXTflex® Unique Dual Index Barcodes -  Unique dual index adapters for multiplexing Illumina® sequencing libraries

NEXTflex® ChIP-Seq Barcodes  -  Unique adapters available in sets of 6, 12, 24, 48 barcodes

NEXTflex-96™ ChIP-Seq Barcodes  -  96 unique adapters available in sets of 96 barcodes

 


RNA-Seq Barcodes for Illumina® Sequencing

These barcodes are ideal for RNA-Seq library prep applications.

 

NEXTflex® Unique Dual Index Barcodes - Unique dual index adapters for multiplexing Illumina® sequencing libraries

NEXTflex® RNA-Seq Barcodes - Unique adapters available in sets of 6, 12, 24, 48 barcodes

NEXTflex-96™ RNA-Seq Barcodes – 96 unique adapters available in sets of 96 barcodes

 


Bisulfite-Seq Barcodes for Illumina® Sequencing

These methylated barcodes are ideal for bisulfite-seq library prep applications.

NEXTflex® Bisulfite-Seq Barcodes – Unique adapters available in sets of 6, 12 and 24 methylated adapters

 


DNA Barcodes for Ion Torrent™ Sequencing

These adapters are ideal for genomic DNA or ChIP-Seq multiplexing applications on Ion Torrent™ sequencing platforms.

NEXTflex® DNA Barcodes for Ion Torrent™ Sequencing – Unique barcodes available in sets of 8, 16, 32 and 64 adapters.