High sensitivity with low detection limit (25 mg/dl)
- Robust kinetic assay
- Rapid – results in 10 minutes
- Does not require expensive instrumentation
- Highly reproducible results
- Measure absorbance in visual spectrum (520 nm)
The MaxDiscovery™ Cholesterol Enzymatic Assay Kit is a plate-based colorimetric enzymatic assay for the determination of cholesterol in serum samples.
The MaxDiscovery Cholesterol Enzymatic Assay Kit uses a coupled enzymatic reaction scheme: cholesterol esters are first converted to cholesterol and fatty acids. Next, cholesterol is oxidized with O2 to form cholesten-3-one + H2O2. Lastly, the hydrogen peroxide is reacted with 4-aminoantipyrine and p-HBS to yield quinoneimine (red dye) and water. The absorption measured at 520 nm, is proportional to the concentration of cholesterol in the sample. The kit also comes with a control solution containing a cholesterol standard (500 mg/dl) which can be used to calibrate the assay.
FORMAT: 1 x 96 well (Removable 8 wells x 12 strips)
MEASUREMENT: Microtiter plate reader (520 nm)
TESTS PER KIT: The kit has the capacity for 96 determinations or testing of 42 samples in duplicate
SHELF LIFE: 3 months when stored under optimal conditions
SAMPLE SIZE: 10 μL/well
SAMPLE TYPES: Rodent Serum